The nucleotide binding oligomerization domain like receptors, or NOD like receptors (NLRs 3 &5), are intracellular receptors responsible for recognizing pathogens in vertebrates. Several NLR mammalian models have been characterized and analyzed but few studies have been performed with teleost species. In this study, we analyzed the nucleotide sequence of six mRNA variants of NLRC3 and NLCRC5 in Atlantic salmon (SsNLRC3), and we deduced the amino acid sequence coding for two different isoforms with a total length of 1135 amino acids and 1093 amino acids. We analyzed the phylogeny of all variants, including a Piscirickettsia salmonis infection in Atlantic salmon. All variants and their expression pattern during infection were analyzed using real-time qPCR. One of the analyzed variants was over-expressed during the early stages of Piscirickettsia salmonis infection, and we were able to identify two different SsNLRC3 isoforms. Lastly, we observed that the pathogen recognition of Piscirickettsia salmonis intracellular infecction can directly modulate inflamasome.
Dr. Yañez is a full professor at the Universidad Austral de Chile. Participate as researcher in FONDAP INCAR. He did his doctoral research at UMKC University, USA. He has published more than 100 research articles, book chapters.