Abstract:

Historically the lumpy skin disease (LSD) was restricted to Africa, but in 2015 was entered for the first time into Balkan region and spreading into several countries, causing serious challenge regarding implementation of successful control of disease and emergence of vaccination. LSD is economically important infectious disease of cattle, caused by LSD virus, genus Capripoxvirus, family Poxviridae. The first case of LSD in Serbia was laboratory confirmed in June 2016 in Municipality of Bujanovac (South part of Serbia). Virus was isolated in MDBK cells and complete genome of LSD virus was determined from the isolate SRB/Bujanovac/2016 by next generation sequencing, using Ion Torrent technology. The sequence of LSD virus SRB/Bujanovac/2016 (KY702007) consists of 150.661 nucleotides and has 99,95% nucleotide identity with virulent South African LSD virus Neethling Warmbaths LW strain (AF409137), isolated in 1999 from severely infected calf. The nucleotide sequence SRB/Bujanovac/2016 is 100% identical with partial gene sequences of G-protein coupled chemokine receptor (GPCR), available for LSD virus strains detected in Turkey and Greece during 2014 and 2015, confirming high nucleotide identity with strains from previously reported outbreaks in neighboring area. The determined virus contains 156 open reading frames (ORFs). The individual nucleotide changes were randomly distributed through whole viral genome. This was the first complete LSD virus genome determined in Serbia and also in Balkan area.

Biography:

Ivan Toplak is an associate professor on Veterinary faculty, University of Ljubljana. His field of interest in research work are epidemiology of especially dangerous diseases and molecular diagnostics of viral diseases. His main research and professional work is in the field of pestiviruses (bovine viral diarrhea virus (BVDV), classical swine fever (CSF) virus, Border disease virus), Aujeszky’s disease virus, African swine fever virus, porcine reproductive and respiratory syndrome (PRRS) virus, swine vesicular disease virus, porcine circovirus type 2, rabies virus, hepatitis E virus (HEV), diagnostics of bee viruses (ABPV, BQCV, CBPV, DWV, SBV), porcine epidemic diarrhea virus (PEDV), Schmallenberg virus, coronaviruses, respiratory viruses in cattle and others.

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