Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as opportunistic agent causing urinary tract infections (UTI), being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is associated to its ability to adhere to host cells and to survive to host immune defenses. Our results show that clinical strains can possess differences in the protein repertoire, specially related to expression of virulence factors. Phenotypic, genotypic and proteomic differences reflect in the ability to survive during interaction with host cells and our data describes proteomic flexibility among S. saprophyticus strains reflecting in virulence and persistence. On the other hand, the proteins secreted by pathogenic microorganisms are the first molecules to interact with the host during infection, for this reason, the secreted antigens represent important targets for the development of diagnostic tests, vaccines and immunotherapies for infectious diseases. Our research group detected – by using immunoproteomic approach – epitopes of B cells from S. saprophyticus secreted proteins. The detection and selection of potential targets for the identification of S. saprophyticus can be used to develop new quickly diagnostic tools for detecting UTI.
Juliana Alves Parente Rocha is a biomedical doctor with a doctorate in Medical Sciences from the University of Brasília. She is an associate professor at the Federal University of Goiás (UFG) in the biochemistry and molecular biology department and a research productivity fellow in Brazil. She has published 25 articles in international journals in the past 5 years. She is currently coordinator of the graduate program in genetics and molecular biology at UFG and one of the coordinators of the COVID- 19 diagnostic campaign laboratory from UFG.